The study of organ preservation morphological changes to the intravenous injection of an Histidine-tryptophan-ketoglutarate solution into amputated limbs

Abstract

Background: Currently, there is no liquid specially used for the preservation and perfusion of the limb in clinics. Nowadays, the transplantation technology of solid organs such as kidney, liver and heart has been relatively mature. Before transplantation, UW fluid perfusion of donor kidney and HTK fluid perfusion of donor heart can achieve good functional protection, which has become an international consensus. Therefore, the need to study the effect of HTK solution on organs is the basis of our research. Aim: To investigate the effect of HTK infusion on the protection and replantation of amputated limb. Materials and Methods: In experiment the amputated limbs of male New Zealand white rabbits were preserved by different preservation methods, including low-temperature HTK solution perfusion group, low-temperature normal saline perfusion group, low-temperature preservation group and non-perfusion group at normal temperature. Bax, Bcl-2 protein immunohistochemical staining and Tunel method were used to detect apoptosis in nerve and vascular tissues. Results: Immunohistochemical staining of tissues (nerve and blood vessel) in each group showed that the number of cells stained by anti-apoptotic protein BCL-2 in each group significantly increased with the extension of time, while the number of cells stained by pro-apoptotic protein BAX2 in each group significantly decreased. Tunel test showed that compared with other treatment groups, the apoptosis rate of HTK liquid group was significantly decreased. Conclusion: Perfusion preservation of severed limbs at 4℃ can effectively reduce tissue damage caused by ischemia and hypoxia, and the effect is stronger than direct preservation. HTK solution and normal saline were used for one-time perfusion of the severed limbs of rabbits, and the preservation effect of HTK solution was the best.