Comparison of the activity of commercial mouthwash solutions against Aggregatibacter actinomycetemcomitans

Abstract

Background: Periodontal disease is one of the most common dental diseases. It is caused by many factors and causes damage to the gums, periodontal bone, conjunctive tissue, and osseous tissue, resulting in tooth loss due to tooth movement. Aggregatibacter actinomycetemcomitans, one of the causative agents of periodontitis, is a highly virulent bacterium that synthesizes leukotoxin (ltxA). Determining the A. actinomycetemcomitans activity of commonly used in Mongolia mouthwash solution in our country is important to increase the therapeutic effect of periodontal disease and prevent it. Aim: To determine the activity of commercial mouthwash solutions commonly used in Mongolia against A. actinomycetemcomitans, ATCC 43718 strain. Materials and Methods: We used to study the A. actinomycetemcomitans ATCC 43718 strain and three different commonly used herbal and chlorhexidine mouthwash solutions with alcohol content. Ethical committee approval was obtained by protocol 2023/3-01. The A. actinomycetemcomitans strain was recultured according to the manufacturer’s instructions, and the antibacterial activity of the antiseptic solution was determined by broth microdilution assay. Statistical processing was done using “SPSS 17.0” software. Results: The A. actinomycetemcomitans ATCC 43718 strain was reconstituted according to the manufacturer’s instructions and cultured clear, convex, round, small adherent colonies in blood media. A broth microdilution assay showed that the above-mentioned mouthwash had antibacterial activity in 15–30 seconds at a 1:4–1:128 dilution. Conclusions: The activity of commonly used mouthwash solutions against standard strains of Aggregatibacter actinomycetemcomitans is acceptable, but results vary depending on the formulation.