The results of studying Babesia microti in some provinces where ticks are prevalent in Mongolia

Abstract

Background: Babesiosis is an infectious disease caused by a single-celled parasite that infects ticks. Blood transfusions are a potential route of transmission for parasites that can cause severe disease in immunocompromised individuals. In this study, we aimed to evaluate the prevalence of Babesia microti infection in blood donors in Khuvsgul, Bulgan, and Selenge provinces, which are the epidemic areas of the tick. Aim: We will check Babesia microti transmission prevalence in Khuvsgul, Bulgan, Selenge province in blood donor Materials and Methods: Blood from a total of 409 donors was collected from the National Transfusion Research Center of Khuvsgul, Bulgan, and Selenge Provinces, and DNA was isolated and directly used for PCR using a Quagen DNA isolation kit. We performed PCR using the KODFxNeo PCR kit. B. microti DNA was taken as a positive control and water as a negative, and the PCR products were subjected to 1.5% agarose gel electrophoresis and the results were evaluated. Results: The life expectancy of the donors in the study was 37 and 58% were women, 42%, 29.3% were first-time donors, and 70.7% were repeat donors. Amplification of 18S RNA gene fragments of B.microti from a total of 409 donors at the Blood Transfusion Center of Khuvsgul, Bulgan and Selenge Provinces Babesia microti disease did not detected. In these states where we live, we see a low risk of B. microti transmission through blood transfusions. Conclusion: Among the blood donors of Khuvsgul, Bulgan and Selenge provinces, B. microti parasite was not detected. The risk of contracting B. microti is relatively low. ‑