Study and determine a pathogen at the level of species oral candidiasis in people who are neck and head cancer

Abstract

Background: Oral fungal infections were reported in 55.2% of the patients who received radiation therapy for neck and head cancer due to decreased saliva production and as a result the amount of oral fungi in oral cavity is increased by 27-77% due to therapy. Candida albicans is the most common of the 150-200 species of fungi that cause oral fungal infections and is found in 60-80% of healthy and sick people. Also C. glabrata, C. krusei, C. parapsilosis, C. dubliniensis, C. tropicalis, C. kefyr, and C. guilliermondii are very common. The basis of the study is to study the drug sensitivity of a pathogen of oral candidiasis in people who are treated with radiation therapy and chemotherapy of neck and head. Aim: The aim of this study is to study and determine a pathogen at the level of oral candidiasis in people who are treated with radiation therapy of neck and head. Materials and Methods: A swab was taken from the morphological elements of the oral mucosa of the study participants who were admitted to the radiation therapy department of National Cancer Center and also those who were treated during the day and placed in transport medium (Amies). Independent colony was isolated carefully from pure cultures grown on candidia chromium agar then was identified and tested for sensitivity to fungal drugs. To do this, 3 ml of saline solution was added to a clean tube then the independent colony of fungi was added to prepare turbid. The turbid should be prepared equal to 2.00 Mc Fairland turbid, which was measured and adjusted with a density. After dipping the tube part of the drug sensitivity card in the prepared fungi turbid, read it on the VITEK II compact device. The results of the study were processed using statistical software Stata-25 and Microsoft Excel, and detailed statistical analysis was performed. Results: After identifying the pathogens of oral fungal infections using VITEK II COMPACT device in case group participants undergoing radiation therapy for head and neck cancer, Candida albicans was present in 75%, Candida dubliniensis in 15%, Candida spherical in 5% and Cryptococcus laurentii in 5% and Candida albicans in 100% of participants in the control group. Conclusion: Oral candidiasis pathogens were found most commonly Candida albicans the participants of case and control group. Analysis revealed that Candida was found in 100% among the control group participants. We conclude that head and neck cancer patients who receiving radio therapy, in particular, were more high incidence oral fungal disease.