Detection of genes coding aminoglycoside modifying enzymes in E. coli isolates from surgically resected appendices

Abstract

Background: Aminoglycoside antibiotics are mainly used to treat infections caused by aerobic Gram-negative bacteria such as E.coli, K.pneumoniae, and P.aeruginosa. Pathogenic microorganisms harbor of aminoglycoside-modifying genes, enabling enzymatic inactivation of aminoglycosides by acetylation, adenylation, or phosphorylation, resulting in elevated resistance to aminoglycoside antibiotics. These genes including Aac (6’)-Ib, Ant (3’) - Ia, Aph (3’)-Ia, Aac (3’) are localized on plasmid with genes beta-lactamases. Thus pathogenics to become resistance to either aminoglycosides and extendedspectrum cephalosporins, resulting in complicated the selection of antibiotics for empirical treatment in infection. Aim: For this reason, we aimed to determine of aminoglycoside resistance genes in E. coli in isolates from surgically resected appendices. Results: In our study result shows that DEC pathogens were detected in 51.4% (55/107) of 107 E.coli samples, identification from surgically resected appendices’ samples which were collected in the bacteriological laboratory of the Second general hospital, between January and June 2021. The Aac II (3’), encoding an acetylating enzyme that induces resistance to gentamicin and tobramycin, was detected 68 strains. The Ant (3’)-Ia gene encoding enzyme that causes resistance to streptomycin, was found in 37 strains, and the Aac(3’)-II gene, and the Ant (3’)-Ia gene, in 27 strains. Acetylating enzyme encoding Aac(6’)-Ib gene, which is resistant to tobramycin and amikacin, and phosphorylating enzyme encoding Aph (3’)-Ia, which is resistant to kanamycin and neomycin, were not detected. Conclusion: Aac (3’)-II gene for acetylating enzyme encoding and Ant (3’)-Ia gene for adenylating enzyme encoding, which is resistant to aminoglycosides, were found in E. coli in isolates from surgically resected appendices.