Determination of anti-inflammatory effects of Dehydrocostus Lactone on lipopolysaccharideinduced CXCL2 gene expression on microglial cells

Abstract

Background: The mechanism of dehydrocostus lactone, a component of Eerdun Wurile, which is widely used in Mongolian Medicine for neurological disease, has not yet been fully studied. Chemokine CXC ligand 2 (CXCL2) is released from macrophages and binds to CCR2 receptors on the cell surface for mobilizing cells, activiting inflammatory processes and regulating the immune system. The study of the interaction between CXCL2 of the microglial cells and dehydrocostus lactone is important to understand dehydrocostus lactone effects. Objective: To study the anti-inflammatory effect of dehydrocostus lactone on CXCL2 gene expression on the microglial cells. Materials and Methods: BV2 microglial cells derived from the mouse brain were cultured in three different conditions: 1. alone, 2. with the lipopolysaccharides, 3. with the dehydrocostus lactone, and then with the addition of lipopolysaccharides. Total RNA was extracted from these three different types of cultured microglia cells to synthesize cDNA, and the level of gene expression of CXCL2 was determined by real-time polymerase chain reaction (qRT-PCR). Results: In the microglial cells, CXCL2 gene expression was detected on the basic activation. Whereas lipopolysaccharides were strongly activated 3 times higher mRNA expression of CXCL2 gene. Interestingly dehydrocostus lactone pre-treatment significantly inhibited lipopolysaccharide-induced CXCL2 gene mRNA expression in the microglial cells. Conclusions: Dehydrocostus lactone inhibits the mRNA expression of CXCL2 gene which is increased by lipopolysaccharides in microglial cells.