The virulence factor genes of Helicobacter pylori study among gastric cancer and non-gastric cancer patients

Abstract

Background: Mongolia ranks first in the world by the incidence and mortality of gastric cancer1. The Helicobacter pylori /H.p/ is considered as the major cause of gastric cancer. Previous studies have shown that the prevalence of H.p infection among Mongolians is around 70%, as in developing countries such as Africa and India and Bangladesh2. Interestingly, although the prevalence of H.p infection is high in these developing countries, the incidence of gastric cancer is low. This can be explained by the differences in the genotype of the CagA toxicity and the genes of the CagPAI assembly. There is no detailed study of the genes of the CagPAI assembly of H.p in Mongolia. Aim: To study of Helicobacter pylori CagPAI gene assembly among gastric cancer and non-gastric cancer patients Materials and methods: A cross-sectional study was conducted among Mongolian adult patients. Gastric endoscopy, gastric histology, H.p isolation, and its gene sequence were performed in gastric cancer and non-gastric cancer cases. The diagnosis of gastric cancer was determined by the Lawrence classification. After culturing H.p infection, the gene sequence of the CagPAI complex genes was determined and statistically processed. Result: The average age of the participants was 48 and sex ratio was 1:1. The 72.22% (n=39) of the participants was non-cancer and the 27.77% (n=15) of the participant diagnosed with cancer. The CagPAI complete genes of Helicobacter pylori was 79.48% (31/39) in non-gastric cancer patients and 100% (15/15) in gastric cancer patients. Conclusion: All 26 genes of the H.pylori CagPAI complex genes determined in Mongolian patients who diagnosed with gastric cancer which indicating that all CagA oncogenic protein is able to be injected to the host cells.