The results of morphometric and gravimetric study of cell damage in of experimental acute infarction model induced by coronary artery occlusion

Abstract

Recently years, Increasing coronary artery disease in the worldwide. Epidemiologists point out that ischemic heart disease is one of the most common complications in the world. In order to develop a basic research methodology for the development of new herbal medicines within the framework of the traditional medicine strategy, a pathological pattern of experimental acute infarction model induced by coronary artery occlusion in rats to determine the presence of respiratory dehydrogenase enzymes in the surrounding ischemic micro circulation, also weight of the dead area heart muscle and size of the heart cavity. Adult male Wistar rats, weighting approximately 200 to 250±20 gram, used for the experiment. We did coronary occlusion induced myocardial ischemia by Kogan A.K., Ambaga M. (1979) method. The experiment is 1st, 3rd, 7th, 14th 21st days Nitro blue tetrazolium staining was used to determine the weight of healthy tissue with bright blue staining or normal cellular respiration, redox potential, proton and electron transport the weight of the unpainted area increased by 9.63-20.34% (p≤0.05). Ischemic standing nodule electron proton leak and lost dehydrogenase enzyme activity.