The effects of khurtsiin deed-6 to the regulation nitric oxide, calcitonin gene-related peptide, endothelin in nitroglycerin induced migraine in rats

Abstract

Background: Migraine is a complex nervous system disease characterized by typical throbbing and unilateral headache, which causes severe healthy and social issues worldwide. The purpose of this study was to investigate the effect of Khurtsiin deed-6 on the treatment of migraine. Material and methods: Sixty rats were randomly divided equally into six groups, including a normal group, model group, Khurtsiin deed-6 50 mg/kg, 100 mg/kg, 150 mg/kg and Ibuprofen tablets 85 mg/kg groups. All rats were intragastrically treated with the corresponding treatment for 10 consecutive days, and they were subcutaneously injected with NTG(10mg/ kg) 1h after the last treatment, except in the normal group. Four hours after NTG treatment, all rats were anaesthetized and the blood was collected. Thereafter, nitric oxide (NO), calcitonin gene-related peptide (CGRP), endothelin (ET) in plasma were detected by enzyme-linked immunosorbent assay method. Results: Compared with the model group, the levels of NO and CGRP in Khurtsiin deed-6 administration groups were markedly decreased (p<0.001), and the levels of ET was no statistcally significant difference. Conclusions: Khurtsiin deed-6 could alleviate the migraine-like headache induced by NTG, which is related to the regulation of vasoactive substances. These findings may contribute to the further study of Khurtsiin deed-6 as a potential drug for migraine pharmacotherapy.