Effect of Vincextoxicum Sibirica on Castor Oil-Induced Diarrheal Disease Model

Abstract

Background: According to an international study every year, 525,000 individuals worldwide die from diarrheal illnesses of various causes. The Indra-4 formula is frequently used in traditional medicine to treat diarrhea, with Holorhena Antidysenterica being one of its main components. However, some practitioners have used Vincextoxicum Sibirica as a substitute, which has not been thoroughly studied. This raises the need to investigate its effects on diarrhea. Aim: To determine the effects of Vincextoxicum Sibirica on castor oil-induced acute enteritis. Materials and Methods: Using the method of Hamidu Usman (2020), 40 Wistar rats weighing 180-220 grams were divided into four groups: healthy, control, comparison, and Vincextoxicum Sibirica group. To cause acute enteritis, castor oil was given to the other groups apart from the healthy and control groups at a dose of 10 ml/kg using a specialized syringe. In addition, PGE2 and TNF-α levels were measured and compared using the ELISA method, and the number of cases of diarrhea and biochemical analyses were conducted over 6 hours. Results: Compared to the control group (76.24±3.60), PGE2 levels of the Vincextoxicum Sibirica group (60.74±0.0) were decreased by 25.5%, whereas PGE2 levels of the Diarex group (72.92±7.81) were reduced by 4.6%. Compared to the control group, the Vincextoxicum Sibirica group exhibited a 28% increase in chloride levels and a 16.1% reduction in potassium levels. Conclusion: In the experimental castor oil-induced acute enteritis model, Vincextoxicum Sibirica was found to lower prostaglandin E2 levels, reducing inflammation and preventing electrolyte and fluid loss.