The effect of Johnsh-4 on the bone structure of osteoporosis model in rats

Abstract

Background: Osteoporosis is the most common bone disease in humans, which damages the support and movement organs, reduces work capacity and is harmful to human health and society. Most of the drugs and preparations for the treatment of this condition are imported, and they have problems such as side effects and high costs. Since there are many drugs used in traditional medicine for bone injuries, traditional fluorine-based drugs are more effective in practice. Aim: The effect of Johnsh-4 on the bone structure of osteoporosis model in rats Materials and Methods: Our research was meticulously carried out, ensuring the highest standards of scientific rigour. We used a normal group, model group, Johns-4 (1:10 decoction, 2.112 g/kg, 1.056 g/kg, 0.528 g/kg), comparison group (1000 mg tablets of CalciD-Denk. 1.322 g/ kg) divided into six groups. SD female rats were carefully selected for the study (8 in each group), and osteoporosis was induced by retinoic acid (70mg/kg). The bone health assessment was conducted using the measurement of bone marrow in experimental rats with osteopenia by D. Tsendjav et al. (2012), and the analysis of bone ash in rats with osteoporosis by the method of Huang Yi Fan (1998) determines the weight of bone ash, the amount of Ca and P contained in it. The statistical analysis was calculated using GraphPad Prism 6.0 and SPSS 23 software, ensuring the accuracy and reliability of the results. Results: The findings of our study are significant. In comparison to the experimental group, the width of the medullary bone in the model group decreased by 11.3% (p<0.05) and the width by 22.7% (p<0.05) in the model group compared to the model group. Four increased by 23% (p<0.05) at the high dose, and only Johnsh-4 increased by 23% (p<0.05) at the high dose, but there was no statistical difference in the length dimension. When comparing the groups, P in the ash of the spinal vertebrae of rats decreased by 46% (p<0.05) in the model group compared to the normal group and increased by 51% (p<0.05) and 41% (p<0.001) in the experimental group compared to the model group, respectively. Results: Compared with the normal group, the narrowness of the medullary bone in the model group decreased by 11.3% (p<0.05) and the wide by 22.7% (p<0.05). Compared to the model group, as for the width of the medullary bone, Jonsh-4 increased by 23% (p<0.05) at the high dose, but there was no statistical difference in the length size. Compared between the groups, P level in the ash of the spinal vertebrae decreased by 46% (p<0.05). In the Jonsh-4, compared to the model group, it increased by 51% (p<0.05) and 41% (p<0.001). In addition, the Ca level in the spinal cord ash of rats was reduced by 50% (p<0.05) in the model group compared to the model group, while it was increased by 49% (p<0.05) and 44% (p<0.05) in the Johnson-4 group compared to the model group. Conclusion: In an experimental animal model of osteoporosis caused by retinoic acid, the Johnsh-4 formula forms a bone matrix and strengthens bones by increasing the weight of medullary bone and the content of calcium and phosphorus in bone ash. The results suggest that traditional medicines containing minerals may have an anti-osteoporosis effect. Therefore, in the future, it is essential to confirm the anti-osteoporosis effect of the Johnsh-4 formula at the level of proteins involved in the signaling pathway of osteoblasts and osteoclast cells, which are bone-forming precursor cells.