Result of laboratory and tissue analysis during Alcoholic liver disease in experimental animals

Abstract

Background: Alcoholic liver disease is fatty liver, hepatitis, liver fibrosis, cirrhosis, such as liver cancer is a widespread disease caused by long-term overdose. However, until now, the clinical treatment methods for ALD are still very limited. Appropriate animal models of ALD are of great importance in determining the pathogenesis of ALD, finding effective therapeutic targets, and evaluating the therapeutic effects of relevant drugs. Aim: Creation of an experimental animal model of Alcoholic liver disease. Materials and Methods: 20 SD male rats weighing 120-180 g were used as experimental animals.The experimental rats were divided into healthy group (n10) and experimental group (n10). In the experimental group of experimental animals, the model of Alcoholic liver diseaser was performed according to the method of Chinese researcher FU Jian-feng (2006). Results: There were statistically significant differences in fibrinogen and APTT in coagulation tests, but no differences were observed in RT at 28 and 56 days of the experiment.The levels of ALT, AST, and GGT in the biochemical analysis were statistically significant (p˂0.05) on the 28th day and 56th day of the experiment.There are statistically significant differences in IL-1, IL-8, and IL-17 levels in the blood plasma of experimental animals.The CK-8 content was insignificant on the 28th day of the experiment and statistically significant on the 56th day. Conclusions: 1. Statistically significant differences in blood coagulation, biochemical and immunological parameters compared to the healthy and experimental groups indicate the occurrence of alcohol-induced chronic inflammation of the liver. 2. In histological analysis, fatty droplets were accumulated in the plasma of liver cells. The ratio of cell plasma and nucleus is lost. The cell nucleus is enlarged and the nucleolus is more visible. There is necrosis with capillary blood filling in the sinusoid.